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Fluidigm: Juno System

Fluidigm Corporation launched an early access program for its new SNP genotyping platform, the JunoTM System, for its key production genomics customers and prospects.

Fluidigm Corporation launched an early access program for its new SNP genotyping platform, the Juno^TM System, for its key production genomics customers and prospects.

The new sample prep engine for SNP genotyping allows scientists to genotype low-concentration DNA samples from tissue, buccal swabs, FFPE, and polyploid organisms in less than three hours.

Traditionally, SNP genotyping uses either microfluidic chips or microtiter plates (which require substantial amounts of reagents resulting in high operating costs), and high-concentration, high-quality samples. The high cost and sample quality constraints hampered the practical use of low-concentration DNA samples, such as buccal swabs and FFPE, in production genomics.

Fluidigm’s new Juno^TM System uses a first of its kind integrated fluidic circuit (IFC) that incorporates preamplification and genotyping of up to 96 samples and 96 assays on a single IFC. To address constraints of traditional SNP genotyping, the new IFC requires as little as 5.5 nanograms of DNA at a concentration of 2.5 nanograms per microliter of sample (human genomic DNA equivalent).

The Juno platform also reduces data collection time while diminishing the risks of error and PCR contamination inherent in multi-step protocols conducted in open-air microtiter plates. While other solutions on the market require multiple hands-on steps and at least five hours from sample to data, the one-step, walk-away Juno System workflow takes just 15 minutes to prep and produces data in less than three hours.

Fluidigm is also releasing new master mix chemistries that combine all of the reagents needed to perform integrated genotyping on the Juno^TM System.

Targeted uses for the Juno System include sample identification, quality control, and DNA fingerprinting by biobanking and cell culture facilities, and integrated on-chip preamplification and genotyping analysis by Agbio customers.

Additionally, the flexibility and ease-of-use of the Juno platform will allow core labs to easily accommodate the needs of many different customers, and the system’s ability to integrate preamplification and genotyping on a single IFC will allow clinical labs to economically provide rapid, accurate results from low-concentration DNA samples.